Regulatory
PsaDintron

Part:BBa_K2984078

Designed by: Dimitri Schumacher   Group: iGEM19_Humboldt_Berlin   (2019-10-19)


PsadIntron - Promoter for enhanced expression


The AR Promoter is composed of the HSP70 promoter fused to the RBCS2 Intron. We followed a smiliar approach fusing the Rbcs2 Intron to the end of Psad promoter , hoping to achieve a higher expression yield. Every CDS assembled with this part would be fused to the Rbcs2 Intron. This part is a part of the Chlamy-HUB-Collection.It is desgined according to MoClo standard carring A1-A3 overhangs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

MoClo Overhangs

This part with A1-A3 MoClo overhangs can be found here.


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Categories
Parameters
None