Composite

Part:BBa_K2799014

Designed by: Jacob Mejlsted, Joen Haahr Jensen, David Faurdal   Group: iGEM18_DTU-Denmark   (2018-10-07)


PtrpC- melA - tCaMV

This BioBrick functions to increase expression of melanin in fungi. It contains melA, which encodes a tyrosinase involved in the first step of melanins biosynthesis, under control of the strong constitutive fungal promoter PtrpC derived from Aspergillus nidulans. It's terminator is derived from the Cauliflower Mosaic Virus (tCaMV).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1693
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1545
    Illegal BsaI.rc site found at 850


Contribution:

The DTU Biobuilders 2018 demonstrated apparent transformants, which suggests integration of this BioBrick into the genome of A. oryzae. The apparent transformants exhibits darker growth than the surrounding area.



[edit]
Categories
//function/reporter/pigment
Parameters
None