Composite

Part:BBa_K2783020

Designed by: Matthijs Jonathan Tadema   Group: iGEM18_Groningen   (2018-10-07)


T7 Endoglucanase II

Endoglucanase II from Trichoderma reesei (EGII) cleaves the beta 1-4 bond between glucose monomers in a cellulose polymer, creating two smaller polymers. This EGII construct includes a dockerin domain, allowing to adhere to an adhesin. A His6-tag was added at the N-terminus of the sequence to allow easy purification. Detailed characterization is available on the page of the parent part BBa_K2783021.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 284
    Illegal AgeI site found at 1049
  • 1000
    COMPATIBLE WITH RFC[1000]


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