Measurement

Part:BBa_K1461234

Designed by: Takefumi Yoshikawa   Group: iGEM14_UT-Tokyo   (2014-09-29)


ecf11 promoter mesurement system (positive feedback)

This part has ecf11 positive feedback system. Arabinose-induction causes ecf11 expression, and once ecf11 expressed, then the positive feedback system is activated and generate ecf11 continually. ecf11 remains in the cell and continue to activate the expression of GFP under ecf11 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1660


[edit]
Categories
Parameters
None