Measurement

Part:BBa_K1461233

Designed by: Takefumi Yoshikawa   Group: iGEM14_UT-Tokyo   (2014-09-29)


ecf20 promoter mesurement system (positive feedback)

This part has ecf20 positive feedback system. Arabinose-induction causes ecf20 expression, and once ecf20 expressed, then the positive feedback system is activated and generate ecf20 continually. ecf20 remains in the cell and continue to activate the expression of GFP under ecf20 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1633


[edit]
Categories
Parameters
None