Measurement

Part:BBa_K1461232

Designed by: Takefumi Yoshikawa   Group: iGEM14_UT-Tokyo   (2014-09-29)


single counter

This device generates mRNA coding GFP whose RBS (ribosome binding site) is covered with cis-repressor (cr) sequence. Arabinose-induction expresses taRNA (BBa_K1461003), which recovers the RBS from crRBS and activates the expression of GFP. This system is basic way to count in our σ-Re Counter project.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1091
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1031
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 745
    Illegal BsaI.rc site found at 1102


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