Project

Part:BBa_K1461230

Designed by: Takefumi Yoshikawa   Group: iGEM14_UT-Tokyo   (2014-09-29)


reset function using ecf11

This part constitutively codes GFP. IPTG-induction extinguishes GFP expression, coding anti ecf11 (BBa_K1461007), which prevents the interaction of ecf11 (BBa_K1461005) and ecf11 promoter (BBa_K1461002).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2657
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2390
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1660


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Parameters
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