Project

Part:BBa_K1461227

Designed by: Takefumi Yoshikawa   Group: iGEM14_UT-Tokyo   (2014-09-29)


resettable single counter system using ecf20

This part can be used to characterize the reset-function of our σ-Re Counter project. Arabinose-induction causes GFP expression, and IPTG-induction extinguishes the GFP expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2147
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2276
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1633


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Categories
Parameters
None