Generator
Part:BBa_K1404002:Design
Designed by: Alexandre Duprey Group: iGEM14_INSA-LYON (2014-09-23)
Ptac-NiCoTB, improves nickel and cobalt internalization
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 857
Illegal AgeI site found at 1056 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This parts associates the strong constitutive promoter Ptac (as found in the plasmid pKK233-2, Clontech), a strong RBS desgined by team Paris Bettencourt 2009, a nickel and cobalt transporter originating from the locus Saro_0344 of Novosphingobium aromaticivorans DSM 12444 which was codon-optimised for expression in E. coli and the bidirectional terminator B0014
Source
The promoter and the rest...