Coding

Part:BBa_K1352010:Design

Designed by: Ana-Maria Cujba, Martyna Sroka   Group: iGEM14_Aberdeen_Scotland   (2014-10-01)
Revision as of 20:53, 1 October 2014 by Registry (Talk | contribs)

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Ag43 autotransporter (ORF)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 709
    Illegal NgoMIV site found at 1933
    Illegal NgoMIV site found at 2443
    Illegal NgoMIV site found at 2464
    Illegal AgeI site found at 1459
    Illegal AgeI site found at 2203
    Illegal AgeI site found at 2617
    Illegal AgeI site found at 2859
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

6 additional PstI restriction sites ( C_TGCA^G) within the open reading frame of Ag43 from BioBrick BBa_K759001 have been removed through the insertion of synonymous mutations (CTGCAA). These were introduced through PCR-based Site-Directed Mutagenesis (Agilent Lightning Quik-Change mutagenesis kit)


Source

PCR amplified from BioBrick BBa_K759001, then subjected to Site-Directed Mutagenesis.

References