Regulatory

Part:BBa_K1059005

Designed by: Xue Sun   Group: iGEM13_OUC-China   (2013-08-29)


DNA segment whose transcript can prevent mRNA degradation by RNaseE.

This segment consists of two RBS,a start codon and several stop codon in different reading frames. We think it will be higher efficient in protecting mRNA from degradation. It is used in the 5'-UTR of mRNA, preventing mRNA degradation mediated by RNaseE.The extra RBS can binding ribosome, preventing RNaseE or the degradosome actived by 5' ribonucleotide.The start codon is for more efficient binding and the stop codon prevents translation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 6
    Illegal NheI site found at 29
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None