Coding
CL2-tag

Part:BBa_K4927004

Designed by: Chuan Liu   Group: iGEM23_HUBU-SKY-China   (2023-10-06)


CL2

Introduction

Our team designed truncated mutants CL7, which were inactivated by E. coli CE7 nuclease. Based on the high similarity of amino acid sequences between CE2, CE8, and CE9 and CE7, corresponding CE2, CE8, and CE9 truncated mutants CL2, CL8, and CL9 were designed, and corresponding inhibitors Im2, Im7, Im8, and Im9 were obtained to construct an artificial protein scaffold Scaf-CIQ.

Figure1:a.CE7/IM7 reaction pairs; B.CE7/DNA reaction pair;C.CE7/CL7 protein sequence alignment


Usage and Biology

The CL2 tag will bind to methanol dehydrogenase (ADH) to form complex A2 and 2A(See the figure below）

Figure2:The strcture of A2


Figure3:The strcture of 2A


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None