Composite

Part:BBa_K4864008

Designed by: Jyothishree V   Group: iGEM23_SVCE-CHENNAI   (2023-10-10)


pAsr-SSP-RBS-Laccase-Linker-CFP

This part consists of a pH-inducible promoter, secretory signal peptide, laccase enzyme-coding sequence, a Tandem GS linker, and a reporter gene. The pH-inducible promoter, Asr (which stands for Acid Shock RNA), gets activated in the pH range of 3.5-5.5. With the help of the secretory signal peptide, the laccase enzyme is secreted extracellularly. In order to confirm the secretion of the laccase enzyme at the laboratory level, we added the reporter gene (CFP) in fusion with the laccase gene using a 13 Tandem GS linker.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 242
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1080
    Illegal BglII site found at 1512
    Illegal BamHI site found at 1889
    Illegal XhoI site found at 1112
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 295
    Illegal NgoMIV site found at 1751
    Illegal NgoMIV site found at 2225
    Illegal AgeI site found at 479
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1034
    Illegal SapI.rc site found at 544


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Categories
Parameters
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