Part:BBa_K4812022

proS

Proline-tRNA ligase (ProRS) is an essential component of the aminoacyl-tRNA synthetases family, which plays a pivotal role in interpreting the genetic code by linking amino acids to their respective tRNA molecules, driven by ATP hydrolysis. Specifically, ProRS falls under the Class IIA aminoacyl-tRNA synthetases. In its solution form, ProRS from E. coli B exists as a dimer.

A comprehensive understanding of ProRS includes its specificity determinants within tRNAPro for recognition, notably the R144 residue, which makes crucial contact with G72 in the tRNAPro acceptor stem. The catalytic activity and efficiency of aminoacylation, particularly in the context of unmodified tRNAPro, have been studied. An experimental evolution experiment revealed how point mutations in ProRS can enhance aminoacylation efficiency, mitigating the growth defect associated with TrmD deficiency.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 460
  Illegal EcoRI site found at 625
  Illegal PstI site found at 508
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 460
  Illegal EcoRI site found at 625
  Illegal PstI site found at 508
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 460
  Illegal EcoRI site found at 625
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 460
  Illegal EcoRI site found at 625
  Illegal PstI site found at 508
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 460
  Illegal EcoRI site found at 625
  Illegal PstI site found at 508
  Illegal AgeI site found at 595
  
• 1000
  COMPATIBLE WITH RFC[1000]