Part:BBa_K4812021
SclB
Sequence of collagen-like protein from Streptococcus pyogenes. GenBank: AJ301806.1
Usage and Biology
The striking and extensive collagen-like structure of the putative SclB protein appears unprecedented amongst the prokaryotic proteins characterized to date. The extensive run of GXYn repeats resembles the remarkably regular structure of collagen, where virtually every third residue is glycine. It is thus tempting to speculate that SclB may exist in the same triple-helix conformation as collagen, composed of three protein units wound around each other to form a stiff cable. Attempts to express the protein in Escherichia coli have so far proved unsuccessful, perhaps reflecting the unusual structure of SclB. SclB shows many similarities to SclA, but also to M and M-like proteins of S. pyogenes. Like SclA and M proteins, SclB has a hypervariable N-terminal part.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 587
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 587
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 587
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 587
Illegal AgeI site found at 454 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 898
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