Composite
Part:BBa_K4800062
Designed by: Yifei Wu Group: iGEM23_NJTech-China-B (2023-10-11)
pRSFDuet-sfp-MmCAR(Q302E)-SpyTag-EutM-SpyCatcher-YahK
The genes of certain protein scaffolds including SpyTag, EutM and SpyCatcher were subcloned into the plasmid pRSFDuet-sfp-MmCAR(Q302E)-YahK together to yield plasmid pRSFDuet-sfp-MmCAR(Q302E)-SpyTag-EutM-SpyCatcher-YahK.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 4393
Illegal EcoRI site found at 8178
Illegal XbaI site found at 1675
Illegal XbaI site found at 8096
Illegal SpeI site found at 3076
Illegal SpeI site found at 3607
Illegal PstI site found at 4828
Illegal PstI site found at 4855
Illegal PstI site found at 6124
Illegal PstI site found at 6151
Illegal PstI site found at 6544
Illegal PstI site found at 7040
Illegal PstI site found at 7684 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4393
Illegal EcoRI site found at 8178
Illegal NheI site found at 8866
Illegal SpeI site found at 3076
Illegal SpeI site found at 3607
Illegal PstI site found at 4828
Illegal PstI site found at 4855
Illegal PstI site found at 6124
Illegal PstI site found at 6151
Illegal PstI site found at 6544
Illegal PstI site found at 7040
Illegal PstI site found at 7684 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4393
Illegal EcoRI site found at 8178
Illegal BglII site found at 5382
Illegal BglII site found at 6008
Illegal BglII site found at 6284
Illegal BglII site found at 6975
Illegal BamHI site found at 4365
Illegal BamHI site found at 7939
Illegal BamHI site found at 8172
Illegal XhoI site found at 7924
Illegal XhoI site found at 8011 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 4393
Illegal EcoRI site found at 8178
Illegal XbaI site found at 1675
Illegal XbaI site found at 8096
Illegal SpeI site found at 3076
Illegal SpeI site found at 3607
Illegal PstI site found at 4828
Illegal PstI site found at 4855
Illegal PstI site found at 6124
Illegal PstI site found at 6151
Illegal PstI site found at 6544
Illegal PstI site found at 7040
Illegal PstI site found at 7684 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 4393
Illegal EcoRI site found at 8178
Illegal XbaI site found at 1675
Illegal XbaI site found at 8096
Illegal SpeI site found at 3076
Illegal SpeI site found at 3607
Illegal PstI site found at 4828
Illegal PstI site found at 4855
Illegal PstI site found at 6124
Illegal PstI site found at 6151
Illegal PstI site found at 6544
Illegal PstI site found at 7040
Illegal PstI site found at 7684
Illegal NgoMIV site found at 6652
Illegal NgoMIV site found at 6714
Illegal NgoMIV site found at 6931
Illegal NgoMIV site found at 7378
Illegal AgeI site found at 4558
Illegal AgeI site found at 5569
Illegal AgeI site found at 5866
Illegal AgeI site found at 6751
Illegal AgeI site found at 6760
Illegal AgeI site found at 7435
Illegal AgeI site found at 10200 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 10273
Illegal SapI site found at 920
[edit]
Categories
Parameters
None |