Composite

Part:BBa_K4800059

Designed by: Yifei Wu   Group: iGEM23_NJTech-China-B   (2023-10-11)


pRSFDuet-sfp-MmCAR(Q302E)-YahK

Based on the plasmid of pRSFDuet-sfp-MmCAR-YahK, we designed point mutation primers and cloned the whole plasmid using PCR. The linearized plasmid was isolated and purified by agarose gel electrophoresis, and then transferred into E. coli Trans-T1 to obtain the targeted plasmids of pRSFDuet-sfp-MmCAR(Q302E)-YahK.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4393
    Illegal XbaI site found at 1675
    Illegal SpeI site found at 3076
    Illegal SpeI site found at 3607
    Illegal PstI site found at 4828
    Illegal PstI site found at 4855
    Illegal PstI site found at 6124
    Illegal PstI site found at 6151
    Illegal PstI site found at 6544
    Illegal PstI site found at 7040
    Illegal PstI site found at 7684
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4393
    Illegal NheI site found at 7952
    Illegal SpeI site found at 3076
    Illegal SpeI site found at 3607
    Illegal PstI site found at 4828
    Illegal PstI site found at 4855
    Illegal PstI site found at 6124
    Illegal PstI site found at 6151
    Illegal PstI site found at 6544
    Illegal PstI site found at 7040
    Illegal PstI site found at 7684
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4393
    Illegal BglII site found at 5382
    Illegal BglII site found at 6008
    Illegal BglII site found at 6284
    Illegal BglII site found at 6975
    Illegal BamHI site found at 4365
    Illegal XhoI site found at 7924
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4393
    Illegal XbaI site found at 1675
    Illegal SpeI site found at 3076
    Illegal SpeI site found at 3607
    Illegal PstI site found at 4828
    Illegal PstI site found at 4855
    Illegal PstI site found at 6124
    Illegal PstI site found at 6151
    Illegal PstI site found at 6544
    Illegal PstI site found at 7040
    Illegal PstI site found at 7684
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4393
    Illegal XbaI site found at 1675
    Illegal SpeI site found at 3076
    Illegal SpeI site found at 3607
    Illegal PstI site found at 4828
    Illegal PstI site found at 4855
    Illegal PstI site found at 6124
    Illegal PstI site found at 6151
    Illegal PstI site found at 6544
    Illegal PstI site found at 7040
    Illegal PstI site found at 7684
    Illegal NgoMIV site found at 6652
    Illegal NgoMIV site found at 6714
    Illegal NgoMIV site found at 6931
    Illegal NgoMIV site found at 7378
    Illegal AgeI site found at 4558
    Illegal AgeI site found at 5569
    Illegal AgeI site found at 5866
    Illegal AgeI site found at 6751
    Illegal AgeI site found at 6760
    Illegal AgeI site found at 7435
    Illegal AgeI site found at 9283
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 9356
    Illegal SapI site found at 920


[edit]
Categories
Parameters
None