Composite
Part:BBa_K4800059
Designed by: Yifei Wu Group: iGEM23_NJTech-China-B (2023-10-11)
pRSFDuet-sfp-MmCAR(Q302E)-YahK
Based on the plasmid of pRSFDuet-sfp-MmCAR-YahK, we designed point mutation primers and cloned the whole plasmid using PCR. The linearized plasmid was isolated and purified by agarose gel electrophoresis, and then transferred into E. coli Trans-T1 to obtain the targeted plasmids of pRSFDuet-sfp-MmCAR(Q302E)-YahK.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 4393
Illegal XbaI site found at 1675
Illegal SpeI site found at 3076
Illegal SpeI site found at 3607
Illegal PstI site found at 4828
Illegal PstI site found at 4855
Illegal PstI site found at 6124
Illegal PstI site found at 6151
Illegal PstI site found at 6544
Illegal PstI site found at 7040
Illegal PstI site found at 7684 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4393
Illegal NheI site found at 7952
Illegal SpeI site found at 3076
Illegal SpeI site found at 3607
Illegal PstI site found at 4828
Illegal PstI site found at 4855
Illegal PstI site found at 6124
Illegal PstI site found at 6151
Illegal PstI site found at 6544
Illegal PstI site found at 7040
Illegal PstI site found at 7684 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4393
Illegal BglII site found at 5382
Illegal BglII site found at 6008
Illegal BglII site found at 6284
Illegal BglII site found at 6975
Illegal BamHI site found at 4365
Illegal XhoI site found at 7924 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 4393
Illegal XbaI site found at 1675
Illegal SpeI site found at 3076
Illegal SpeI site found at 3607
Illegal PstI site found at 4828
Illegal PstI site found at 4855
Illegal PstI site found at 6124
Illegal PstI site found at 6151
Illegal PstI site found at 6544
Illegal PstI site found at 7040
Illegal PstI site found at 7684 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 4393
Illegal XbaI site found at 1675
Illegal SpeI site found at 3076
Illegal SpeI site found at 3607
Illegal PstI site found at 4828
Illegal PstI site found at 4855
Illegal PstI site found at 6124
Illegal PstI site found at 6151
Illegal PstI site found at 6544
Illegal PstI site found at 7040
Illegal PstI site found at 7684
Illegal NgoMIV site found at 6652
Illegal NgoMIV site found at 6714
Illegal NgoMIV site found at 6931
Illegal NgoMIV site found at 7378
Illegal AgeI site found at 4558
Illegal AgeI site found at 5569
Illegal AgeI site found at 5866
Illegal AgeI site found at 6751
Illegal AgeI site found at 6760
Illegal AgeI site found at 7435
Illegal AgeI site found at 9283 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 9356
Illegal SapI site found at 920
[edit]
Categories
Parameters
None |