Composite
Part:BBa_K4800054
Designed by: Chenxi Ma Group: iGEM23_NJTech-China-B (2023-10-09)
pTrc99a-davB-davA-GabT
We use the laboratory conserved plasmid pET22b-T7-davB-T7-davA as templates, the fragments of davB and davA were amplified by PCR with a consensus RBS and Ptrc respectively. Subsequently, the Ptrc-RBS-davB-Ptrc-RBS-davA fragment was obtained by overlap PCR, which was then ligated into the linearized vector pTrc99a-GabT by In-fusion cloning method to obtain plasmid pTtrc99a-davB-davA-GabT.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 5122
Illegal XbaI site found at 6489
Illegal PstI site found at 6501 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 5122
Illegal PstI site found at 6501
Illegal NotI site found at 2808 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 5122
Illegal BglII site found at 3897 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 5122
Illegal XbaI site found at 6489
Illegal PstI site found at 6501 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 5122
Illegal XbaI site found at 6489
Illegal PstI site found at 6501
Illegal NgoMIV site found at 4057
Illegal AgeI site found at 3464
Illegal AgeI site found at 3713
Illegal AgeI site found at 5535 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 6685
Illegal BsaI site found at 7753
Illegal SapI.rc site found at 767
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Categories
Parameters
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