Composite
Part:BBa_K4800053
Designed by: Chenxi Ma Group: iGEM23_NJTech-China-B (2023-10-09)
pACYCDuet-sfp-MmCAR-YahK
We used codon-optimized and synthesized to obtain the fragment of sfp-MmCAR, which was then ligated into the pACYCDuet plasmid under the control of trc promoter. The Ptrc-YahK fragment was amplified with plasmid pTrc99a-YahK as the template, and In-Fusion cloning was then used to ligate into the above vectors to yield plasmid pACYCDuet-sfp-MmCAR-YahK.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 4801
Illegal XbaI site found at 2084
Illegal PstI site found at 5236
Illegal PstI site found at 5263
Illegal PstI site found at 6532
Illegal PstI site found at 6559
Illegal PstI site found at 6952
Illegal PstI site found at 7448
Illegal PstI site found at 8092 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4801
Illegal NheI site found at 1243
Illegal NheI site found at 8360
Illegal PstI site found at 5236
Illegal PstI site found at 5263
Illegal PstI site found at 6532
Illegal PstI site found at 6559
Illegal PstI site found at 6952
Illegal PstI site found at 7448
Illegal PstI site found at 8092 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4801
Illegal BglII site found at 5790
Illegal BglII site found at 6416
Illegal BglII site found at 6692
Illegal BglII site found at 7383
Illegal BamHI site found at 4773
Illegal XhoI site found at 8332 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 4801
Illegal XbaI site found at 2084
Illegal PstI site found at 5236
Illegal PstI site found at 5263
Illegal PstI site found at 6532
Illegal PstI site found at 6559
Illegal PstI site found at 6952
Illegal PstI site found at 7448
Illegal PstI site found at 8092 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 4801
Illegal XbaI site found at 2084
Illegal PstI site found at 5236
Illegal PstI site found at 5263
Illegal PstI site found at 6532
Illegal PstI site found at 6559
Illegal PstI site found at 6952
Illegal PstI site found at 7448
Illegal PstI site found at 8092
Illegal NgoMIV site found at 7060
Illegal NgoMIV site found at 7122
Illegal NgoMIV site found at 7339
Illegal NgoMIV site found at 7786
Illegal AgeI site found at 57
Illegal AgeI site found at 1329
Illegal AgeI site found at 1652
Illegal AgeI site found at 4966
Illegal AgeI site found at 5977
Illegal AgeI site found at 6274
Illegal AgeI site found at 7159 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |