Composite

Part:BBa_K4800052

Designed by: Qiming Zou   Group: iGEM23_NJTech-China-B   (2023-10-08)


pTrc99a-YahK-QLH

To compare the activity of different transaminases, the DNA fragments of Ptrc-RBS-YahK were amplified and ligated into the plasmids of pTrc99a-QLH.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3624
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4351
    Illegal NgoMIV site found at 4900
    Illegal AgeI site found at 4380
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5283
    Illegal BsaI site found at 6351
    Illegal SapI.rc site found at 767


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