Composite
Part:BBa_K4800051
Designed by: Qiming Zou Group: iGEM23_NJTech-China-B (2023-10-08)
pTrc99a-YahK-SpuC
We linearized the pTrc99a-YahK vector using restriction endonuclease and connected the target gene SpuC to it through homologous recombination to obtain the recombinant plasmid pTrc99a-YahK-SpuC.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 4846
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 4846
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3624
Illegal XhoI site found at 5140 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 4846
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 4846
Illegal AgeI site found at 4129
Illegal AgeI site found at 4582 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 5346
Illegal BsaI site found at 6414
Illegal SapI.rc site found at 767
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Categories
Parameters
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