Composite
Part:BBa_K4800049
Designed by: Chenxi Ma Group: iGEM23_NJTech-China-B (2023-10-08)
pTrc99a-YahK-Spo3471
We linearized the pTrc99a-YahK vector using restriction endonuclease and connected the target gene Spo3471 to it through homologous recombination to obtain the recombinant plasmid pTrc99a-YahK-Spo3471.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 3808
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 3808
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3624
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 3808
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 3808
Illegal NgoMIV site found at 4468
Illegal AgeI site found at 4455
Illegal AgeI site found at 4579 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 5346
Illegal BsaI site found at 6414
Illegal SapI.rc site found at 767
[edit]
Categories
Parameters
None |