Composite
Part:BBa_K4800047
Designed by: Chenxi Ma Group: iGEM23_NJTech-China-B (2023-10-08)
pTrc99a-YahK-GabT
We linearized the pTrc99a-YahK vector using restriction endonuclease and connected the target gene GabT to it through homologous recombination to obtain the recombinant plasmid pTrc99a-YahK-GabT.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3624
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 4039
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 5172
Illegal BsaI site found at 6240
Illegal SapI.rc site found at 767
[edit]
Categories
Parameters
None |