Composite
Part:BBa_K4800045
Designed by: Chenxi Ma Group: iGEM23_NJTech-China-B (2023-10-08)
pTrc99a-SpuC
We linearized the pTrc99a vector using restriction endonuclease and connected the target gene SpuC to it through homologous recombination to obtain the recombinant plasmid pTrc99a-SpuC.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 4051
Illegal PstI site found at 3704
Illegal PstI site found at 4063 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 3704
Illegal PstI site found at 4063 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4045
Illegal XhoI site found at 3998 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 4051
Illegal PstI site found at 3704
Illegal PstI site found at 4063 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 4051
Illegal PstI site found at 3704
Illegal PstI site found at 4063
Illegal AgeI site found at 2987
Illegal AgeI site found at 3440 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4247
Illegal BsaI site found at 5315
Illegal SapI.rc site found at 767
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Categories
Parameters
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