Composite
Part:BBa_K4800043
Designed by: Chenxi Ma Group: iGEM23_NJTech-China-B (2023-10-08)
pTrc99a-SAV2585
We linearized the pTrc99a vector using restriction endonuclease and connected the target gene SAV2585 to it through homologous recombination to obtain the recombinant plasmid pTrc99a-SAV2585.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 4486
Illegal PstI site found at 3174
Illegal PstI site found at 4498 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 3174
Illegal PstI site found at 4498 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4480
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 4486
Illegal PstI site found at 3174
Illegal PstI site found at 4498 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 4486
Illegal PstI site found at 3174
Illegal PstI site found at 4498
Illegal NgoMIV site found at 2710
Illegal NgoMIV site found at 3374
Illegal NgoMIV site found at 3403
Illegal NgoMIV site found at 3448
Illegal NgoMIV site found at 4252
Illegal NgoMIV site found at 4366
Illegal AgeI site found at 3059
Illegal AgeI site found at 3110
Illegal AgeI site found at 3185
Illegal AgeI site found at 3299 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4682
Illegal BsaI site found at 5750
Illegal SapI.rc site found at 767
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Categories
Parameters
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