Composite
Part:BBa_K4800042
Designed by: Chenxi Ma Group: iGEM23_NJTech-China-B (2023-10-08)
pTrc99a-Spo3471
We linearized the pTrc99a vector using restriction endonuclease and connected the target gene Spo3471 to it through homologous recombination to obtain the recombinant plasmid pTrc99a-Spo3471.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 4051
Illegal PstI site found at 2666
Illegal PstI site found at 4063 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 2666
Illegal PstI site found at 4063 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4045
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 4051
Illegal PstI site found at 2666
Illegal PstI site found at 4063 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 4051
Illegal PstI site found at 2666
Illegal PstI site found at 4063
Illegal NgoMIV site found at 3326
Illegal AgeI site found at 3313
Illegal AgeI site found at 3437 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4247
Illegal BsaI site found at 5315
Illegal SapI.rc site found at 767
[edit]
Categories
Parameters
None |