Composite
Part:BBa_K4800038
Designed by: Chenxi Ma Group: iGEM23_NJTech-China-B (2023-10-08)
pTrc99a-Hsd36
We linearized the pTrc99a vector using restriction endonuclease and connected the target gene Hsd36 to it through homologous recombination to obtain the recombinant plasmid pTrc99a-Hsd36.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 3353
Illegal PstI site found at 3365 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 3365
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3347
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 3353
Illegal PstI site found at 3365 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 3353
Illegal PstI site found at 3365
Illegal AgeI site found at 2611
Illegal AgeI site found at 2683
Illegal AgeI site found at 3292 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3549
Illegal BsaI site found at 4617
Illegal SapI.rc site found at 767
[edit]
Categories
Parameters
None |