Composite
Part:BBa_K4800036
Designed by: Yao Yao Group: iGEM23_NJTech-China-B (2023-10-08)
pRSFDuet-MAB4714
We linearized the pRSFDuet vector using restriction endonuclease and connected the target gene MAB4714 to it through homologous recombination to obtain the recombinant plasmid pRSFDuet-MAB4714.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1415
Illegal XbaI site found at 7185
Illegal PstI site found at 7197 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7233
Illegal PstI site found at 7197 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3574
Illegal BamHI site found at 7179 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1415
Illegal XbaI site found at 7185
Illegal PstI site found at 7197 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1415
Illegal XbaI site found at 7185
Illegal PstI site found at 7197
Illegal NgoMIV site found at 4052
Illegal NgoMIV site found at 4354
Illegal NgoMIV site found at 4544
Illegal NgoMIV site found at 4907
Illegal NgoMIV site found at 5962
Illegal NgoMIV site found at 6341
Illegal NgoMIV site found at 6566
Illegal AgeI site found at 3872
Illegal AgeI site found at 4091
Illegal AgeI site found at 5114
Illegal AgeI site found at 5834
Illegal AgeI site found at 6388
Illegal AgeI site found at 7608 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 7429
Illegal BsaI site found at 7681
Illegal SapI site found at 660
[edit]
Categories
Parameters
None |