Composite
Part:BBa_K4800035
Designed by: Yao Yao Group: iGEM23_NJTech-China-B (2023-10-08)
pRSFDuet-MSMEG5739
We linearized the pRSFDuet vector using restriction endonuclease and connected the target gene MSMEG5739 to it through homologous recombination to obtain the recombinant plasmid pRSFDuet-MSMEG5739.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1415
Illegal XbaI site found at 7230
Illegal PstI site found at 7242 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 7242
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 4705
Illegal BamHI site found at 3574
Illegal BamHI site found at 7224 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1415
Illegal XbaI site found at 7230
Illegal PstI site found at 7242 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1415
Illegal XbaI site found at 7230
Illegal PstI site found at 7242
Illegal NgoMIV site found at 3823
Illegal NgoMIV site found at 4418
Illegal NgoMIV site found at 4426
Illegal NgoMIV site found at 4838
Illegal NgoMIV site found at 6938
Illegal AgeI site found at 4565
Illegal AgeI site found at 4883
Illegal AgeI site found at 6121
Illegal AgeI site found at 7653 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 7474
Illegal BsaI site found at 7726
Illegal SapI site found at 660
[edit]
Categories
Parameters
None |