Composite
Part:BBa_K4800034
Designed by: Qiming Zou Group: iGEM23_NJTech-China-B (2023-10-08)
pRSFDuet-MpCAR
We linearized the pRSFDuet vector using restriction endonuclease and connected the target gene MpCAR to it through homologous recombination to obtain the recombinant plasmid pRSFDuet-MpCAR.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1415
Illegal XbaI site found at 7170
Illegal PstI site found at 7182 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 7182
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 4321
Illegal BglII site found at 4609
Illegal BglII site found at 5244
Illegal BglII site found at 5530
Illegal BamHI site found at 3574
Illegal BamHI site found at 7164 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1415
Illegal XbaI site found at 7170
Illegal PstI site found at 7182 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1415
Illegal XbaI site found at 7170
Illegal PstI site found at 7182
Illegal NgoMIV site found at 4327
Illegal NgoMIV site found at 4345
Illegal NgoMIV site found at 4712
Illegal NgoMIV site found at 4895
Illegal AgeI site found at 3857
Illegal AgeI site found at 4169
Illegal AgeI site found at 4274
Illegal AgeI site found at 5102
Illegal AgeI site found at 5459
Illegal AgeI site found at 5984
Illegal AgeI site found at 6794 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 7414
Illegal BsaI site found at 7666
Illegal SapI site found at 660
[edit]
Categories
Parameters
None |