Composite
Part:BBa_K4800019
Designed by: Yifei Wu Group: iGEM23_NJTech-China-B (2023-10-06)
EutM-SpyCatcher
We constructed EutM-SpyCatcher building blocks and attached itself covalently via isopeptide bond formation by the SpyTag-SpyCatcher system to the formed scaffolds,joining with enzyme elements to form a complex and improving the stability of the enzyme element.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 42
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 42
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 42
Illegal BamHI site found at 36 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 42
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 42
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |