DNA
Part:BBa_K4800011
Designed by: Yifei Wu Group: iGEM23_NJTech-China-B (2023-10-06)
YcjQ Donor
YcjQ Donor is exogenous DNA used by cells as a template for homologous recombination repair (HDR) after SgRNA and Cas9 complexes have caused DNA double-strand breaks at the target site.
Build:
Fig1.YcjQ Donor
The genes at both end of the YcjQ gene were selected as the upper and downer homology arms, which were amplified separately, purified and recovered, then joined by overlap to obtain the YcjQ Donor.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 631
Illegal AgeI site found at 1313
Illegal AgeI site found at 1321 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1373
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Categories
Parameters
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