DNA
Part:BBa_K4800010
Designed by: Wenbin Zhao Group: iGEM23_NJTech-China-B (2023-10-06)
ackA-pta Donor
AckA-pta Donor is exogenous DNA used by cells as a template for homologous recombination repair (HDR) after SgRNA and Cas9 complexes have caused DNA double-strand breaks at the target site.
Build:
Fig1.ackA-pta Donor
The genes at both end of the ackA-pta gene were selected as the upper and downer homology arms, which were amplified separately, purified and recovered, then joined by overlap to obtain the ackA-pta Donor.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 969
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 969
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 969
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 969
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |