Composite
Part:BBa_K4800005
Designed by: Jiali Wang Group: iGEM23_NJTech-China-B (2023-10-06)
MmCAR(Q302E)-SpyTag
Binding of MmCAR and SpyTag via linker protein.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 427
Illegal PstI site found at 454
Illegal PstI site found at 1723
Illegal PstI site found at 1750
Illegal PstI site found at 2143
Illegal PstI site found at 2639
Illegal PstI site found at 3283 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 427
Illegal PstI site found at 454
Illegal PstI site found at 1723
Illegal PstI site found at 1750
Illegal PstI site found at 2143
Illegal PstI site found at 2639
Illegal PstI site found at 3283 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 981
Illegal BglII site found at 1607
Illegal BglII site found at 1883
Illegal BglII site found at 2574
Illegal BamHI site found at 3538
Illegal XhoI site found at 3523
Illegal XhoI site found at 3610 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 427
Illegal PstI site found at 454
Illegal PstI site found at 1723
Illegal PstI site found at 1750
Illegal PstI site found at 2143
Illegal PstI site found at 2639
Illegal PstI site found at 3283 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 427
Illegal PstI site found at 454
Illegal PstI site found at 1723
Illegal PstI site found at 1750
Illegal PstI site found at 2143
Illegal PstI site found at 2639
Illegal PstI site found at 3283
Illegal NgoMIV site found at 2251
Illegal NgoMIV site found at 2313
Illegal NgoMIV site found at 2530
Illegal NgoMIV site found at 2977
Illegal AgeI site found at 157
Illegal AgeI site found at 1168
Illegal AgeI site found at 1465
Illegal AgeI site found at 2350
Illegal AgeI site found at 2359
Illegal AgeI site found at 3034 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |