DNA

Part:BBa_K4693008

Designed by: Vibhavari Medepalli   Group: iGEM23_IISER-TVM   (2023-10-10)


cDNA Truncated

This is a short complementary DNA sequence that binds to our 14-mer truncated cortisol aptamer (BBa_K4693008). Together with the aptamer, it forms an aptasensor that can quantify cortisol. The cDNA (complementary ssDNA) was designed to be complementary to half the length of the aptamer, making the cDNA 7bp in length. The delta G value upon binding to the aptamer is -7.3 kcal/mol

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None