Regulatory

Part:BBa_K4651019

Designed by: Jason Gao   Group: iGEM23_DNHS-SanDiego-CA   (2023-10-10)


Modified BBa_K4651007 with -10 and -35 sigma factors changed to match BBa_K4651017

BBa_K4651007 that was modified so that the -10 and -35 sigma factors of the promoter was changed to match those of BBa_K4651017. Below are links to comparisons between iGEM1a (BBa_K4651018), iGEM1b (BBa_K4651019), and iGEM1c (BBa_K4651017) with and without IPTG. The signal is meant to increase with TPA concentration and be dependent on the presence of IPTG. https://drive.google.com/file/d/1Xj8crBkolOILaINQBtPw5ykQMC2e3SaN/view?usp=sharing (with IPTG) https://drive.google.com/file/d/1-uc_nCZ_6gy3wUl6VnnORar75NKFcVsh/view?usp=sharing (without IPTG)

A comparison for only iGEM1b (BBa_K4651019, this part) with and without IPTG: https://drive.google.com/file/d/1LYUs9r4K5RHc1tctbC2_-HQ_C2giiC-T/view?usp=sharing.

A comparison between plasmid without iGEM1b (called mEmerald, which is BBa_K4651001, because that was the reporter used) and plasmid with iGEM1b, both with and without IPTG: https://drive.google.com/file/d/1nZZ9Jh9QNsJLXDWfRRPSClIfd_iloZdW/view?usp=sharing.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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