Part:BBa_K4651019
Modified BBa_K4651007 with -10 and -35 sigma factors changed to match BBa_K4651017
BBa_K4651007 that was modified so that the -10 and -35 sigma factors of the promoter was changed to match those of BBa_K4651017. Below are links to comparisons between iGEM1a (BBa_K4651018), iGEM1b (BBa_K4651019), and iGEM1c (BBa_K4651017) with and without IPTG. The signal is meant to increase with TPA concentration and be dependent on the presence of IPTG. https://drive.google.com/file/d/1Xj8crBkolOILaINQBtPw5ykQMC2e3SaN/view?usp=sharing (with IPTG) https://drive.google.com/file/d/1-uc_nCZ_6gy3wUl6VnnORar75NKFcVsh/view?usp=sharing (without IPTG)
A comparison for only iGEM1b (BBa_K4651019, this part) with and without IPTG: https://drive.google.com/file/d/1LYUs9r4K5RHc1tctbC2_-HQ_C2giiC-T/view?usp=sharing.
A comparison between plasmid without iGEM1b (called mEmerald, which is BBa_K4651001, because that was the reporter used) and plasmid with iGEM1b, both with and without IPTG: https://drive.google.com/file/d/1nZZ9Jh9QNsJLXDWfRRPSClIfd_iloZdW/view?usp=sharing.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
None |