Regulatory

Part:BBa_K4651018

Designed by: Jason Gao   Group: iGEM23_DNHS-SanDiego-CA   (2023-10-10)


BBa_K4651007 modified to be truncated to remove native RBS

BBa_K4651007 that was modified to remove the native RBS in it. Below are links to comparisons between iGEM1a (BBa_K4651018, or this part), iGEM1b (BBa_K4651019), and iGEM1c (BBa_K4651017) with and without IPTG. The signal is meant to increase with TPA concentration and be dependent on the presence of IPTG. https://drive.google.com/file/d/1Xj8crBkolOILaINQBtPw5ykQMC2e3SaN/view?usp=sharing (with IPTG) https://drive.google.com/file/d/1-uc_nCZ_6gy3wUl6VnnORar75NKFcVsh/view?usp=sharing (without IPTG)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None