Composite

Part:BBa_K4616929

Designed by: Haoyu Zhang   Group: iGEM23_BIT-China   (2023-10-03)

Optimized production lines of santalol in S.cerevisiae

Usage

Optimized production lines of santalol in S.cerevisiae.

Biology

The downstream genes sass (alpha-santalene synthase from santalum album), cyp76, and cprs were expressed normally to generate the enzymes SasS(alpha-santalene synthase from Santalum album), CYP76(cytochrome P450 monooxygenase) and CPRs(cytochrome P450 reductase). The corresponding enzymes convert farnesyl pyrophosphate (FPP) to β-Santalene by STS(santalene/bergamotene synthase), and then to β-santalol by CYP76 and CPRs, realizing the goal of converting the precursor FPP to santalol[1].

The basic part contains three basic components, including sass(BBa_K4616003), cyp76(BBa_K4616004) and cprs (BBa_K4616009). For details, please check the specific information of the relevant components.

igem23-part-santalol-biosynthetic.png

Figure 1. Biosynthetic pathway of santalenes and santalols in planta. STS: santalene/bergamotene synthase; CYP: cytochrome P450 monooxygenase; CPR: cytochrome P450 reductase.

Reference

[1]Zha, Wen-long, Zi, Jia-chen. Advances in biotechnological production of santalenes and santalols. Chinese Herbal Medicines, 2021, 13, 90-97.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 466
    Illegal EcoRI site found at 1618
    Illegal EcoRI site found at 4727
    Illegal EcoRI site found at 4775
    Illegal XbaI site found at 2011
    Illegal XbaI site found at 2557
    Illegal XbaI site found at 2887
    Illegal XbaI site found at 4723
    Illegal XbaI site found at 5110
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 466
    Illegal EcoRI site found at 1618
    Illegal EcoRI site found at 4727
    Illegal EcoRI site found at 4775
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 466
    Illegal EcoRI site found at 1618
    Illegal EcoRI site found at 4727
    Illegal EcoRI site found at 4775
    Illegal BglII site found at 2287
    Illegal BglII site found at 4207
    Illegal BglII site found at 4594
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 466
    Illegal EcoRI site found at 1618
    Illegal EcoRI site found at 4727
    Illegal EcoRI site found at 4775
    Illegal XbaI site found at 2011
    Illegal XbaI site found at 2557
    Illegal XbaI site found at 2887
    Illegal XbaI site found at 4723
    Illegal XbaI site found at 5110
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 466
    Illegal EcoRI site found at 1618
    Illegal EcoRI site found at 4727
    Illegal EcoRI site found at 4775
    Illegal XbaI site found at 2011
    Illegal XbaI site found at 2557
    Illegal XbaI site found at 2887
    Illegal XbaI site found at 4723
    Illegal XbaI site found at 5110
    Illegal AgeI site found at 460
  • 1000
    COMPATIBLE WITH RFC[1000]


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