DNA

Part:BBa_K4616169

Designed by: Cheng Zou   Group: iGEM23_BIT-China   (2023-10-03)

lpps is a precursor of sclareol.

Usage and Biology

The lpps gene is positioned downstream of the Pgal1-s(BBa_K4616168).In the presence of blue light, it becomes active and facilitates the expression and translation of enzymes for sclareol synthesis[1].

Reference

[1] Cao, Xuan , et al. "Engineering yeast for high-level production of diterpenoid sclareol." Metabolic engineering (2023).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 67
    Illegal EcoRI site found at 448
    Illegal EcoRI site found at 1648
    Illegal XbaI site found at 19
    Illegal XbaI site found at 973
    Illegal XbaI site found at 1015
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 67
    Illegal EcoRI site found at 448
    Illegal EcoRI site found at 1648
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 67
    Illegal EcoRI site found at 448
    Illegal EcoRI site found at 1648
    Illegal BglII site found at 1678
    Illegal BglII site found at 2102
    Illegal BglII site found at 2138
    Illegal BamHI site found at 1607
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 67
    Illegal EcoRI site found at 448
    Illegal EcoRI site found at 1648
    Illegal XbaI site found at 19
    Illegal XbaI site found at 973
    Illegal XbaI site found at 1015
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 67
    Illegal EcoRI site found at 448
    Illegal EcoRI site found at 1648
    Illegal XbaI site found at 19
    Illegal XbaI site found at 973
    Illegal XbaI site found at 1015
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1952


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Categories
Parameters
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