Regulatory
Part:BBa_K4616168
Designed by: Cheng Zou Group: iGEM23_BIT-China (2023-10-03)
Pgal1-s is a common strongly inducible promoter
Usage:
Pgal1-s is highly inducible in the presence of galactose and the absence of glucose, which will turn on the expression of downstream genes.
Biology:
Pgal1-s activates the expression of downstream genes, including those related to sclareol synthesis under blue light control. Additionally, Pgal1-s functions as a reverse promoter in a route associated with santalol synthesis. It yields a complementary structure with PTEF1-transcribed mRNA under blue light irradiation, thereby preventing santalol synthesis.
Figure 1. PCR result. M-marker.1-Pgal1-s(part K4616168,719 bp).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 8
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 394
Illegal AgeI site found at 605 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |