Part:BBa_K4616012
Fluorescent protein detection gene circuitry in E.coli
Usage
This is one of the E.coli fluorescent protein detection gene circuits in the BIT-China 2023 IGEM project "Light's control iT".
Figure 1. E.coli transformation experimental verification result. M-marker. 1-PBLrep_rbs34_mCherry (composite part K4616012,765 bp).
Biology
When el222 gene is expressed,the photosensitive transcription factor EL222 is generated. Without 450 nm light illumination, the photosensitive transcription factor EL222 stop dimerization and then the downstream gene PBLrep functions as a promoter, then the transcript of gene mCherry starts. mCherry is a red fluorescent protein from mushroom coral, which is a various of essential reporter in cell and molecular biology.
Figure 2.Light control system in E.coli
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 760
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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