Composite

Part:BBa_K4616010

Designed by: Haoyu Zhang   Group: iGEM23_BIT-China   (2023-10-10)

A 450nm light control system in E.coli

Usage

BIT-China team's 2023 IGEM project "Light's control iT" is to be able to control the ratio of sclareol and santalol arbitrarily under the condition of light control in S. cerevisiae, so we designed an interesting light control element, which is the most critical light control part.

Biology

In E.coli, the constitutive promoter initiates so that the downstream el222 gene is expressed, then the photosensitive transcription factor EL222 is generated. Under 450 nm light condition, the photosensitive transcription factor EL222 undergoes homodimerization and then binds to the PBLind sequence, which activates the transcription and expression of the downstream target gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 296
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 296
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 296
    Illegal XhoI site found at 932
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 296
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 296
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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