DNA

Part:BBa_K4616009

Designed by: Lingtao Huang   Group: iGEM23_BIT-China   (2023-10-03)

cprs transcribes the enzyme necessary for santalol production

Usage

After expressing cprs and cyp76(BBa_K4616004), the enzymes catalyze the conversion of santalene into santalol.

Biology

The NADPH-dependent cytochrome P450 reductase (CPRS) ultimately hydroxylates C12 in santalene to yield santalol in collaboration with CYP76[1].

igem23-part-sass-cprs2.png

Figure 1. PCR result. M-marker. 2-cprs (part K4616009, 2157bp).

Reference

[1]Wang, Yuchen , et al. "Progress in biosynthesis of santalene and santalol." Sheng wu gong cheng xue bao = Chinese journal of biotechnology 34.6(2018):862-875.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1487
    Illegal EcoRI site found at 1535
    Illegal XbaI site found at 1483
    Illegal XbaI site found at 1870
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1487
    Illegal EcoRI site found at 1535
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1487
    Illegal EcoRI site found at 1535
    Illegal BglII site found at 967
    Illegal BglII site found at 1354
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1487
    Illegal EcoRI site found at 1535
    Illegal XbaI site found at 1483
    Illegal XbaI site found at 1870
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1487
    Illegal EcoRI site found at 1535
    Illegal XbaI site found at 1483
    Illegal XbaI site found at 1870
  • 1000
    COMPATIBLE WITH RFC[1000]


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