Regulatory

Part:BBa_K4616005

Designed by: Zexu Wang   Group: iGEM23_BIT-China   (2023-10-03)


a promoter controlled by el222(K4616138).

Usage

Pc120 is a promoter regulated by el222(BBa_K4616138). When EL222 functions as a transcription factor, Pc120 facilitates the expression of downstream genes, thus establishing the fundamental framework of the photocontrol system. Finally, the genes synthesizing sclareol are expressed under blue light irradiation.

Biology

EL222 is a photosensitive transcription factor comprising two functional domains. One is the Light-Oxygen-Voltage (LOV) sensing domain, which responds to light at 450nm, leading to homologous dimerization of EL222. The second domain is the Helix-Turn-Helix (HTH) DNA-binding domain, which specifically recognizes and binds to the C120 repeat sequence[1].

Reference

[1]Zhao, et al. "Optogenetic regulation of engineered cellular metabolism for microbial chemical production." Nature (2018).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 453
    Illegal NotI site found at 14
    Illegal NotI site found at 441
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None