Part:BBa_K4616004

cyp76 transcribes the enzyme necessary for santalol production

Usage

cyp76 transcribes the enzyme necessary for santalol production.

Biology

cyp450 has the capability to hydroxylate carbon at different positions using various terpenes as substrates. As a result, it can catalyze the hydroxylation of C12 in santalene to produce santalol. Many scholars have cloned multiple cyp450 genes from S. album by analyzing and mining the transcriptomic database inyieldation. Most of these genes have the ability to catalyze the conversion of sandalene to santalol, with Sa_CYP76_F39v1 exhibiting a catalytic rate close to 100%[1].

Figure 1. PCR result. M-marker. 1-cyp76 (part K4616004,1546 bp).

Reference

[1] Wang, Yuchen, et al. "Progress in biosynthesis of santalene and santalol." Sheng wu gong cheng xue bao = Chinese journal of biotechnology 34.6(2018):862-875.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 286
  Illegal XbaI site found at 832
  Illegal XbaI site found at 1162
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 562
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 286
  Illegal XbaI site found at 832
  Illegal XbaI site found at 1162
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 286
  Illegal XbaI site found at 832
  Illegal XbaI site found at 1162
  
• 1000
  COMPATIBLE WITH RFC[1000]