Part:BBa_K4616003
sass transcribes the enzyme necessary for santalol production
Usage
When the sass gene is attached to the back of the pTEF1(BBa_K4616002) promoter, in the absence of blue light, the mRNA transcribed by this gene will not be unable to express due to the yieldation of complementary structures, so the enzyme necessary for the synthesis of santalol will be translated.
Biology
Misra et al. reported that all tissues (including callus, germ, seed, old tree and young tree) of S.album contain SaSS enzyme. The extracted protein can catalyze the synthesis of products by FPP in vitro experiments, consistent with the primary components of essential oil. Subsequently, the SasS gene was cloned from S.album, S.Alstrocaledonicum and S.picatum. Sequence determination, heterologous expression, and functional verification were carried out.
Figure 1. PCR result. M-marker. 1-sass (part K4616003,1757 bp).
References
[1] Wang, Yuchen , et al. "Progress in biosynthesis of santalene and santalol." Sheng wu gong cheng xue bao = Chinese journal of biotechnology 34.6(2018):862-875.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 466
Illegal EcoRI site found at 1618 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 466
Illegal EcoRI site found at 1618 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 466
Illegal EcoRI site found at 1618 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 466
Illegal EcoRI site found at 1618 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 466
Illegal EcoRI site found at 1618
Illegal AgeI site found at 460 - 1000COMPATIBLE WITH RFC[1000]
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