Part:BBa_K4616002

The P_TEF1 promoter

Usage

P_TEF1 is a strong constitutive promoter. As a constitutive promoter, P_TEF1 can efficiently initiate the expression of the downstream el222 gene, thus initiating the operation of the entire blue-light-controlled system. Additionally, P_TEF1 can directly activate the expression of genes associated with santalol synthesis.

Biology

P_TEF1, compared with another strong constitutive promoter, the glyceraldehyde-3-phosphate dehydrogenase promoter (pGAP).P_TEF1 has been found to exhibit favorable peryieldance in high-glucose environments and under carbon restriction conditions, resulting in similar or even higher expression levels of recombinant proteins[1]. Notably, P_TEF1 operates as a constitutive promoter and does not require inducers. The study suggests that P_TEF1 offers a wider range of production conditions and more straightforward expression methods than P_GAP. Therefore, P_TEF1 is an excellent choice for expressing exogenous gene in Pichia Pastoris for industrial applications.

Reference

[1]唐瑞琪等. "酿酒酵母人工杂合启动子与天然启动子活性比较." 生物技术通报 33.1(2017):9.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 167