Coding

Part:BBa_K4195018

Designed by: Honglin Song   Group: iGEM22_XMU-China   (2022-09-26)


araC

AraC is an operon of arabinose promoter (BBa_I0500).

Biology

In the absence of arabinose, the AraC dimer adopts a different conformation and binds to araO2 and araI1. In this position, there is no monomer at site araI2, and so the protein cannot activate the araBAD promoter (araPBAD). When arabinose is present, arabinose binds to AraC, changing the shape of that activator so that it binds as a dimer to araI1 and araI2. This places one monomer of AraC close to the promoter from which it can activate transcription.

Usage

We planned to add another araC gene after BBa_I0500 in order to achieve the stricter regulation of this inducible promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None