Coding

Part:BBa_K4119042

Designed by: Chenchen Xu   Group: iGEM22_NJTech_China   (2022-10-11)


pMTL-PpchA-pchA-Plee1

butyrate-biosensor pMTL-PpchA-pchA-Plee1


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1654
    Illegal EcoRI site found at 2663
    Illegal XbaI site found at 2736
    Illegal SpeI site found at 4897
    Illegal PstI site found at 2
    Illegal PstI site found at 2775
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1654
    Illegal EcoRI site found at 2663
    Illegal NheI site found at 2954
    Illegal SpeI site found at 4897
    Illegal PstI site found at 2
    Illegal PstI site found at 2775
    Illegal NotI site found at 81
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1654
    Illegal EcoRI site found at 2663
    Illegal BglII site found at 573
    Illegal BglII site found at 2763
    Illegal BamHI site found at 2730
    Illegal XhoI site found at 2767
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1654
    Illegal EcoRI site found at 2663
    Illegal XbaI site found at 2736
    Illegal SpeI site found at 4897
    Illegal PstI site found at 2
    Illegal PstI site found at 2775
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1654
    Illegal EcoRI site found at 2663
    Illegal XbaI site found at 2736
    Illegal SpeI site found at 4897
    Illegal PstI site found at 2
    Illegal PstI site found at 2775
    Illegal NgoMIV site found at 5402
    Illegal NgoMIV site found at 7309
    Illegal NgoMIV site found at 7319
    Illegal NgoMIV site found at 7445
    Illegal AgeI site found at 1487
    Illegal AgeI site found at 2024
    Illegal AgeI site found at 2147
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1554
    Illegal SapI.rc site found at 7061
    Illegal SapI.rc site found at 7416
    Illegal SapI.rc site found at 7787


[edit]
Categories
Parameters
None