Generator

Part:BBa_K4054108

Designed by: Xinyan Han   Group: iGEM21_XJTLU-CHINA   (2021-10-19)


pLuxR_T7 RBS_Rep_LuxR

This is a composite part used for luxR expression. It consist of pLuxR, T7 RBS and ther coding sequence of reporter GFP As it include the component "pLuxR", the activator LuxR is required to start the transcription. Meanwhile, the RepA-luxR expressed by it slef can also induce the expression, which lead to a self-regulation.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 314
    Illegal PstI site found at 764
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 901
    Illegal PstI site found at 764
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 314
    Illegal PstI site found at 764
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 314
    Illegal PstI site found at 764
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None